Physicochemical characterization of human intestinal lactase
نویسندگان
چکیده
منابع مشابه
Neonatal intestinal lactase activity.
The sequential changes in intestinal lactase activity of 40 neonates were measured indirectly from the differential uptake and excretion of lactose and the non-metabolisable disaccharide lactulose contained in formula feeds. A daily decline in urinary lactose:lactulose excretion ratios, reflecting a rise in intestinal lactase activity, followed formula feeding. Percentage decline was related di...
متن کاملThe Lactase Activity of the Intestinal Mucosa of the Dog and Some Characteristics of Intestinal Lactase
The early work on intestinal lactase has been reviewed by Plimmer (1906) and Oppenheimer (1925). No uniformity of opinion was reached regarding the presence and dist,ribution of this enzyme in the intestinal mucosa or in intestinal juice. Without attempting to review these investigations again, it suffices to state that some authors found lactase in the small intestine of young animals. Others ...
متن کاملImproved purification of rat intestinal lactase.
A rapid and improved method to obtain purified lactase from rat intestine is described. The purification procedure involved only two chromatographic steps. The degree of purification was far above (500 fold) the values reached with classical methods. Rabbit antisera raised to the purified lactase were characterized using conventional immunological techniques. The specificity of the lactase anti...
متن کاملStudies on the expression of intestinal lactase in different individuals.
Sixty one duodenal biopsy specimens were examined for the expression of lactase at the level of enzyme activity, protein, and messenger RNA. Of the 51 samples with normal villous architecture, 39 were lactase persistent, 11 were nonpersistent (adult type hypolactasia), and one was of indeterminate status. All the lactase persistent individuals showed high mRNA and a high level of the lactase pr...
متن کاملMechanism of maturational decline of rat intestinal lactase-phlorizin hydrolase.
The maturational decline in lactase-phlorizin hydrolase (LPH) activity was studied in groups of young rats ranging from suckling to early post-weaned states. Associated maturational increases in sucrase-isomaltase (SI) and maltase-glucoamylase (MG) activities were also examined as a comparison. Over this time period changes in cellular concentrations of the three enzymes were observed, reflecti...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1987
ISSN: 0264-6021,1470-8728
DOI: 10.1042/bj2410567